Abstract
We have fractionated human tonsillar B cells on the basis of CD5 expression and determined the nucleotide sequences of immunoglobin light chain variable (V) regions encoded by the single member of the V chi 4 gene family in both CD5+ and CD5- populations. The majority of cDNA from both CD5+ and CD5- B cells populations harbored somatic mutations. Thus, human tonsillar CD5+ B cells, unlike their murine counterparts, are capable of activating their somatic hypermutation mechanism, resulting in the accumulation of somatic mutation in the VL regions.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Antigens, CD / analysis
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B-Lymphocyte Subsets / metabolism*
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Base Sequence
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CD5 Antigens
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Genes, Immunoglobulin*
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Humans
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Immunoglobulin Variable Region / genetics
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Immunoglobulin kappa-Chains / genetics*
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Molecular Sequence Data
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Mutation
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Oligodeoxyribonucleotides / chemistry
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Palatine Tonsil / cytology
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Polymerase Chain Reaction
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Sequence Alignment
Substances
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Antigens, CD
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CD5 Antigens
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Immunoglobulin Variable Region
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Immunoglobulin kappa-Chains
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Oligodeoxyribonucleotides
Associated data
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GENBANK/S62210
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GENBANK/S62890
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GENBANK/X68697
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GENBANK/X68698
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GENBANK/X72719
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GENBANK/Z14164
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GENBANK/Z14165
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GENBANK/Z14166
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GENBANK/Z14167
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GENBANK/Z34893