Abstract
Recombinant human granulocyte colony-stimulating factor (rhG-CSF) not only enhanced the growth of HL-60 cells, but also significantly increased NBT-reducing ability and alkaline phosphatase (ALP) activity of the cells, which were enhanced by the treatment with retinoic acid (RA). Protein kinase C inhibitors (H-7 and staurosporine) significantly suppressed this induction of ALP. The pretreatment with RA followed by rhG-CSF treatment showed almost the same degree of ALP activity as that induced by the simultaneous treatment with RA and rhG-CSF. This study suggests that RA and rhG-CSF are the potent inducers of ALP activity of HL-60 cells and protein kinase C is supposed to have a role in this induction of ALP.
MeSH terms
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1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
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Alkaline Phosphatase / biosynthesis*
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Alkaloids / pharmacology
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Calcitriol / pharmacology
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Cell Line
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Enzyme Induction / drug effects
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Granulocyte Colony-Stimulating Factor / pharmacology*
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Humans
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Isoquinolines / pharmacology
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Kinetics
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Leukemia, Promyelocytic, Acute / enzymology*
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Piperazines / pharmacology
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Protein Kinase C / antagonists & inhibitors
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Recombinant Proteins / pharmacology
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Staurosporine
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Tretinoin / pharmacology*
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Tumor Cells, Cultured
Substances
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Alkaloids
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Isoquinolines
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Piperazines
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Recombinant Proteins
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Granulocyte Colony-Stimulating Factor
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Tretinoin
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1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
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Protein Kinase C
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Alkaline Phosphatase
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Calcitriol
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Staurosporine