Abstract
Expression of the human immunodeficiency virus type 1 (HIV-1) receptor CD4 on many nonhuman and some human cell lines is not sufficient to permit HIV-1 infection. We describe a human glioblastoma cell line (U373-MG) which remains resistant to HIV-1 despite the added expression of an authentic CD4 molecule. The block to HIV-1 infection of these cells is strain independent and appears to be at viral entry. Heterokaryons of CD4-expressing U373-MG (U373-CD4) cells fused to HeLa cells allow HIV-1 entry. A U373-CD4/HeLa hybrid clone allows efficient HIV-1 replication. These results suggest that HeLa cells express a factor(s) that can complement the viral entry defect of U373-CD4 cells and is necessary for efficient CD4-mediated HIV-1 infection.
Publication types
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Comparative Study
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Antigens, CD / biosynthesis
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Antigens, CD / isolation & purification
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Antigens, CD / metabolism*
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Base Sequence
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Blotting, Western
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CD4 Antigens / biosynthesis
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CD4 Antigens / isolation & purification
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CD4 Antigens / metabolism*
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Cell Fusion
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Electrophoresis, Polyacrylamide Gel
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Flow Cytometry
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Glioma
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HIV Reverse Transcriptase
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HIV-1 / physiology*
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HeLa Cells
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Humans
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Hybrid Cells
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Molecular Sequence Data
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Neoplasm Proteins / isolation & purification
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Neoplasm Proteins / metabolism
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Oligodeoxyribonucleotides
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Polymerase Chain Reaction
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RNA-Directed DNA Polymerase / analysis
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RNA-Directed DNA Polymerase / metabolism
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Recombinant Proteins / analysis
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Recombinant Proteins / biosynthesis
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Tumor Cells, Cultured
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Virus Replication*
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beta-Galactosidase / analysis
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beta-Galactosidase / biosynthesis
Substances
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Antigens, CD
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CD4 Antigens
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Neoplasm Proteins
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Oligodeoxyribonucleotides
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Recombinant Proteins
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HIV Reverse Transcriptase
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RNA-Directed DNA Polymerase
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beta-Galactosidase