Terpenes enhance metabolic activity and alter expression of adhesion molecules (Mac-1 and L-selectin) on human granulocytes

Inflammation. 1993 Aug;17(4):499-509. doi: 10.1007/BF00916589.

Abstract

Granulocytes from healthy blood donors were exposed to terpenes dissolved in ethanol. Flow cytometry was used to measure the expression of the cell surface receptors Mac-1 L-selectin, and CR1, which were detected by monoclonal antibodies. The phagocytic activity was determined by using C3b-coated yeast particles as prey. The metabolic activity was measured by determining the intracellular hydrogen peroxide production using dichlorofluorescein diacetate. After terpene exposure the expression of Mac-1 and CR1 increased (P < 0.001 and P < 0.01, respectively) and the expression of L-selectin decreased (P < 0.001). There was also an increased metabolic activity (P < 0.001). The cell viability, the cell count, and the phagocytic activity remained unchanged. These findings suggest that terpene exposure triggers the granulocytes to a higher degree of activation in terms of an altered expression of the adhesion molecules and an increased metabolic activity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Adhesion
  • Cell Adhesion Molecules / metabolism*
  • Cell Count / drug effects
  • Cell Survival / drug effects
  • Granulocytes / cytology
  • Granulocytes / drug effects
  • Granulocytes / metabolism*
  • Humans
  • L-Selectin
  • Macrophage-1 Antigen / metabolism*
  • Phagocytes / physiology
  • Receptors, Complement 3b / metabolism
  • Terpenes / pharmacology*

Substances

  • Cell Adhesion Molecules
  • Macrophage-1 Antigen
  • Receptors, Complement 3b
  • Terpenes
  • L-Selectin