Hippocampal synaptogenesis in hydrocephalic HTX-rats using a monoclonal anti-synaptic vesicle protein antibody

Brain Dev. 1994 Nov-Dec;16(6):432-6. doi: 10.1016/0387-7604(94)90002-7.

Abstract

The fluorescence intensity (FI) on immunohistochemical staining with a monoclonal antibody (Mab 171 B5) against a synaptic vesicle-associated protein (SVP-38) was measured in the CA1 sector of the hippocampus of brains obtained from hydrocephalic and non-hydrocephalic HTX-rats aged 1, 7, 14, 21 and 28 days. FI was significantly increased on the 21st and 28th postnatal days in hydrocephalic rats as compared to in non-hydrocephalic rats, despite the presence of marked morphological stretching and rotation of the dendritic arborization of Golgi-impregnated neurons in the CA1 sector. The increase in FI appeared to indicate an increase in synaptic density. As previous reports have indicated a marked reduction in SVP-38 FI in the thin cerebral cortex of 28th postnatal day hydrocephalic brains, our findings may suggest that hippocampal synaptogenesis is more resilient than that of the cerebral cortex in hydrocephalic brains.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal
  • Cerebral Cortex / pathology
  • Cerebral Cortex / physiopathology
  • Fluorescent Antibody Technique
  • Hippocampus / pathology
  • Hippocampus / physiopathology*
  • Hydrocephalus / pathology
  • Hydrocephalus / physiopathology*
  • Male
  • Rats
  • Synapses / physiology*
  • Synapsins / biosynthesis*
  • Synapsins / immunology

Substances

  • Antibodies, Monoclonal
  • Synapsins