Reductase activity of aldehyde oxidase toward the carcinogen N-hydroxy-2-acetylaminofluorene and the related hydroxamic acids

Biochem Mol Biol Int. 1994 Dec;34(6):1197-203.

Abstract

Liver aldehyde oxidase (EC 1.2.3.1) was capable of reducing N-arylacetohydroxamic acids, N-hydroxy-2-acetyl-aminofluorene, N-hydroxy-4-acetylaminobiphenyl and N-hydroxyphenacetin, to the corresponding amides in the presence of an electron donor of the enzyme under anaerobic conditions. When supplemented with an electron donor of the enzyme, a significant reduction of N-hydroxy-2-acetylaminofluorene occurred, which was sensitive to an inhibitor of the enzyme. These observations were made with cytosolic fractions prepared from the livers of rabbits, guinea pigs, rats and mice.

Publication types

  • Comparative Study

MeSH terms

  • Aldehyde Oxidase
  • Aldehyde Oxidoreductases / antagonists & inhibitors
  • Aldehyde Oxidoreductases / metabolism*
  • Aldehydes
  • Aminobiphenyl Compounds / metabolism*
  • Animals
  • Carcinogens / metabolism
  • Guinea Pigs
  • Hydroxamic Acids / metabolism*
  • Hydroxyacetylaminofluorene / metabolism*
  • Liver / enzymology*
  • Liver / metabolism
  • Mice
  • Oxidation-Reduction
  • Phenacetin / analogs & derivatives*
  • Phenacetin / metabolism
  • Rabbits
  • Rats
  • Vitamin K / pharmacology

Substances

  • Aldehydes
  • Aminobiphenyl Compounds
  • Carcinogens
  • Hydroxamic Acids
  • Vitamin K
  • N-hydroxyphenacetin
  • N-hydroxy-4-acetylaminobiphenyl
  • Hydroxyacetylaminofluorene
  • Aldehyde Oxidoreductases
  • Aldehyde Oxidase
  • Phenacetin
  • butyraldehyde