Abstract
We have studied the liver 15-hydroxyeicosatetraenoic acid (15-HETE) and leukotriene B4 (LTB4) levels in streptozotocin- (ST)-induced diabetes in rats using liquid chromatography and radioimmunological techniques. Diabetic rats showed significant alterations of liver lipoxygenase metabolites when compared to controls. These 15-HETE and LTB4 increases were concomitant with raised levels of plasma and tissue thromboxane B2 (TXB2) and also urinary 2,3-dinor-TXB2 in plasma and urine, respectively. These changes confirm an activation of 5- and 15-lipoxygenase in the liver 3 days after i.p. ST administration.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Arachidonate 15-Lipoxygenase / metabolism*
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Arachidonate 5-Lipoxygenase / metabolism*
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Arachidonic Acid / metabolism*
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Blood Glucose / analysis
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Diabetes Mellitus, Experimental / metabolism*
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Hydroxyeicosatetraenoic Acids / biosynthesis*
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Liver / enzymology*
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Male
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Rats
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Rats, Wistar
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Streptozocin
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Thromboxane B2 / analogs & derivatives
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Thromboxane B2 / biosynthesis
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Thromboxane B2 / blood
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Thromboxane B2 / urine
Substances
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Blood Glucose
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Hydroxyeicosatetraenoic Acids
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Arachidonic Acid
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Thromboxane B2
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Streptozocin
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2,3-dinor-thromboxane B2
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15-hydroxy-5,8,11,13-eicosatetraenoic acid
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Arachidonate 15-Lipoxygenase
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Arachidonate 5-Lipoxygenase