Hydrogen peroxide at concentrations from 0.1 to 20 microM enhances phagocytosis and oxidative burst of murine peritoneal macrophages. The activation of these macrophage functions is paralleled by prolonged hyperpolarization and a transient increase in cytoplasmic free calcium concentration. All the effects are dose- and time-dependent. The results obtained for H2O2 are compared with those for a natural activator, peptide N-formyl-methionyl-leucyl-phenylalanine. The data demonstrate the ability of small doses of hydrogen peroxide to stimulate macrophages through the intracellular mechanisms of ion transduction.