Abstract
As examined by the luciferase assay, a negative regulatory region including the NF kappa B motif was found in the 5'-flanking region of the 12-lipoxygenase gene in human erythroleukemia cells. The negative control was abolished by a site-specific mutation of the NF kappa B motif. Probes including the NF kappa B region gave positive bands upon a gel-shift assay. The bands were super-shifted by antibodies for NF kappa B p50, NF kappa B p65 and c-Rel, and were lost by a NF kappa B competitor DNA. Furthermore, the NF kappa B sequence was protected in DNase I footprinting. Thus, two kinds of heterodimer (p50 and p65; p50 and c-Rel) seemed to control the over-expression of the human 12-lipoxygenase gene.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Arachidonate 12-Lipoxygenase / genetics*
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Base Sequence
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Cell Nucleus / chemistry
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Gene Expression Regulation, Enzymologic / drug effects*
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Humans
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Leukemia, Erythroblastic, Acute / enzymology
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Luciferases / genetics
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Macromolecular Substances
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Molecular Sequence Data
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NF-kappa B / chemistry
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NF-kappa B / pharmacology*
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Proto-Oncogene Proteins / chemistry
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Proto-Oncogene Proteins / pharmacology*
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Proto-Oncogene Proteins c-rel
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Recombinant Fusion Proteins
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Regulatory Sequences, Nucleic Acid
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Transcription Factors / pharmacology
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Transcription, Genetic / drug effects*
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Tumor Cells, Cultured
Substances
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Macromolecular Substances
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NF-kappa B
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Proto-Oncogene Proteins
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Proto-Oncogene Proteins c-rel
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Recombinant Fusion Proteins
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Transcription Factors
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Arachidonate 12-Lipoxygenase
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Luciferases