Abstract
A cDNA encoding subunit 12 (S12) of human erythrocyte 26 S protease has been isolated, sequenced and expressed. The cDNA contains an open reading frame that encodes a 36.6 kDA protein 96% identical to mouse Mov-34 and 67% identical to its Drosophila melanogaster homolog. Based on the high degree of sequence identity between human S12, mouse and Drosophila Mov-34 proteins, we conclude that the Mov-34 gene product is a component of the 26 S protease. Antibodies produced against two S12 fragments, Met1-Tyr95 (S12f95) and Met1-Leu205 (S12f205), react with S12 transferred to nitrocellulose from SDS-PAGE. In contrast, after transfer from native gels, the epitope(s) recognized by anti-S12f205 is exposed in the regulatory complex but appears to be masked when the regulatory complex associates with the multicatalytic protease.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Cloning, Molecular*
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DNA, Complementary / chemistry
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Drosophila melanogaster
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Erythrocytes / enzymology*
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Gene Expression*
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Humans
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Immunoblotting
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Intracellular Signaling Peptides and Proteins
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Molecular Sequence Data
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Open Reading Frames
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Peptide Hydrolases / chemistry
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Peptide Hydrolases / genetics*
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Polymerase Chain Reaction
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Proteasome Endopeptidase Complex*
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Protein Subunits / chemistry
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Proteins*
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RNA-Binding Proteins
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Recombinant Proteins / chemistry
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Retroviridae Proteins / chemistry
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Sequence Alignment
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Sequence Homology
Substances
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DNA, Complementary
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Intracellular Signaling Peptides and Proteins
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PSMD7 protein, human
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Protein Subunits
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Proteins
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RNA-Binding Proteins
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Recombinant Proteins
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Retroviridae Proteins
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Psmd7 protein, mouse
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Peptide Hydrolases
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Proteasome Endopeptidase Complex
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ATP dependent 26S protease