Abstract
Epstein-Barr virus (EBV) type B, a less potent transformer of B lymphocytes than type A, has rarely been detected in EBV-associated neoplasms except in AIDS-related lymphomas, in which about 50% of the cases contained this sub-type. In this study we analyzed the association of EBV and the distribution of virus sub-types in Asian non-Hodgkin's lymphoma (NHL) of the upper aerodigestive tract. We studied archival material of 29 NHL cases from Malaysia. B- and T-cell associated antigens were demonstrated by immunohistochemistry, and EBV early RNA EBER-1 was demonstrated using the RNA in situ hybridization technique. EBV was detected in the majority of tumour cells in 11/13 T-NHL but in only 1/16 B-NHL. EBV was sub-typed by single-step polymerase chain reaction of the EBNA-2 gene. This was successful in 9/10 cases of EBER-1-positive tumours and all contained type-A virus only. Our results showed a preponderance of T-cell lymphoma of the upper aerodigestive tract in the ethnic Chinese group of Malaysian patients, and EBV was strongly associated with T-NHL but not with B-NHL. Our results suggest that type-A EBV is the prevalent sub-type in Asian NHL of the upper aerodigestive tract, similarly to findings in Asian nasopharyngeal carcinoma.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult
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Aged
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Antigens, CD / analysis
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Antigens, Viral / genetics
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Base Sequence
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Child, Preschool
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DNA Primers
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DNA-Binding Proteins / genetics
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Epstein-Barr Virus Nuclear Antigens
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Female
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Herpesvirus 4, Human / genetics
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Herpesvirus 4, Human / isolation & purification*
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Humans
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In Situ Hybridization
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Incidence
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Lymphoma, Non-Hodgkin / epidemiology
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Lymphoma, Non-Hodgkin / immunology
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Lymphoma, Non-Hodgkin / pathology
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Lymphoma, Non-Hodgkin / virology*
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Malaysia / epidemiology
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Male
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Middle Aged
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Molecular Sequence Data
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Nasopharyngeal Neoplasms / epidemiology
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Nasopharyngeal Neoplasms / immunology
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Nasopharyngeal Neoplasms / pathology
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Nasopharyngeal Neoplasms / virology*
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Oropharyngeal Neoplasms / epidemiology
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Oropharyngeal Neoplasms / immunology
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Oropharyngeal Neoplasms / pathology
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Oropharyngeal Neoplasms / virology*
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Palatal Neoplasms / epidemiology
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Palatal Neoplasms / immunology
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Palatal Neoplasms / pathology
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Palatal Neoplasms / virology*
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Paranasal Sinus Neoplasms / epidemiology
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Paranasal Sinus Neoplasms / immunology
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Paranasal Sinus Neoplasms / pathology
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Paranasal Sinus Neoplasms / virology*
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Polymerase Chain Reaction
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Retrospective Studies
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Trans-Activators / genetics
Substances
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Antigens, CD
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Antigens, Viral
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DNA Primers
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DNA-Binding Proteins
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Epstein-Barr Virus Nuclear Antigens
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Trans-Activators