76Br-labeled monoclonal anti-CEA antibodies for radioimmuno positron emission tomography

Nucl Med Biol. 1995 Jan;22(1):125-31. doi: 10.1016/0969-8051(94)e0065-q.

Abstract

For the application of anti-tumor monoclonal antibodies (MAbs) in positron emission tomography (PET), labeling radionuclides with half-lives allowing a suitable time frame for imaging are required. The anti-CEA MAb 38S1 was labeled with the positron emitting nuclide 76Br (t1/2 16 h) using bromoperoxidase (BPO), and subsequently affinity purified. A procedure was devised to allow reproducible production of MAb-preparations of high immunoreactivity and with acceptable bromination yield. The biological activity of 76Br-38S1 was retained and comparable to that of chloramine-T labeled 125I-38S1, as tested in vitro.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Monoclonal*
  • Bromine Radioisotopes*
  • Carcinoembryonic Antigen / immunology*
  • Humans
  • Isotope Labeling*
  • Radioimmunodetection*
  • Tomography, Emission-Computed*
  • Tumor Cells, Cultured

Substances

  • Antibodies, Monoclonal
  • Bromine Radioisotopes
  • Carcinoembryonic Antigen