Selection of chromosome-specific primers and their use in simple and double PRINS techniques for rapid in situ identification of human chromosomes

Cytogenet Cell Genet. 1995;70(1-2):138-42. doi: 10.1159/000134079.

Abstract

The PRimed IN Situ labeling (PRINS) technique is an alternative to in situ hybridization for rapid chromosome screening. We have defined and tested new specific oligonucleotide primers for alpha-satellite DNA of several chromosomes. When using a semiautomatic PRINS protocol, specific labeling was obtained in both metaphase cells and interphase nuclei in a 1-h reaction. PRINS may be a simple and reliable technique for rapidly detecting aneuploidies.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Chromosomes, Human / genetics*
  • Chromosomes, Human, Pair 2
  • DNA Primers*
  • DNA Probes
  • DNA, Satellite / genetics
  • Humans
  • In Situ Hybridization, Fluorescence / methods*
  • Molecular Sequence Data

Substances

  • DNA Primers
  • DNA Probes
  • DNA, Satellite