A microdissection library of the rat renal carcinoma gene region

Cytogenet Cell Genet. 1995;70(1-2):92-4. doi: 10.1159/000133999.

Abstract

Predisposition to hereditary renal carcinoma in the Eker rat involves a mutation of a putative tumor suppressor gene within chromosome band 10q12. We describe the identification of three unique polymorphic sequences in the vicinity of this locus following the microdissection, construction and characterization of a region-specific DNA library for rat chromosome band 10q12.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Blotting, Southern
  • Carcinoma, Renal Cell / genetics*
  • Chromosome Mapping
  • Dissection / methods
  • Gene Library*
  • In Situ Hybridization, Fluorescence
  • Kidney Neoplasms / genetics*
  • Micromanipulation
  • Polymerase Chain Reaction
  • Rats