Abstract
Two mutant strains, M35 and M89, were obtained by UV irradiation from a wild-type Bacillus subtilis producing iturin and surfactin. Sporulation and surfactin production were similar in both mutants and in the parent strain, while the iturin production of M35 was 300-fold less than that of the wild-type strain; M89 did not produce any iturin. The analysis of the incorporation of sodium [1-14C]acetate into cellular lipids and lipopeptides showed that M89 still synthesized beta-amino fatty acids, the lipid moiety of iturin.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anti-Bacterial Agents / biosynthesis
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Antifungal Agents / biosynthesis
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Bacillus subtilis / genetics
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Bacillus subtilis / metabolism*
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Bacterial Proteins / biosynthesis
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Bacterial Proteins / genetics
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Hemolysis
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Humans
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In Vitro Techniques
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Lipopeptides
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Mutation
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Peptides*
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Peptides, Cyclic / biosynthesis*
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Peptides, Cyclic / genetics
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Spores, Bacterial / genetics
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Spores, Bacterial / metabolism
Substances
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Anti-Bacterial Agents
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Antifungal Agents
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Bacterial Proteins
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Lipopeptides
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Peptides
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Peptides, Cyclic
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surfactin peptide
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iturin A