Influence of iron depletion on growth kinetics, siderophore production, and protein expression of Staphylococcus aureus

FEMS Microbiol Lett. 1995 Apr 1;127(3):195-9. doi: 10.1111/j.1574-6968.1995.tb07473.x.

Abstract

Growth rates, siderophore secretion, and bacterial proteins of two clinical isolates of Staphylococcus aureus were studied over 72 h of growth in iron-supplemented and iron-restricted chemically defined media. Under iron restriction the growth rates were decreased to different extents depending on the strain. Production of siderophore was detected in the mid-exponential and stationary phases of growth. The expression of iron-regulated proteins of 81, 23, and 17 kDa was time-dependent, associated with the same stage of growth, and might be involved in siderophore efficiency.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Outer Membrane Proteins / biosynthesis*
  • Bacterial Outer Membrane Proteins / chemistry
  • Bacterial Outer Membrane Proteins / isolation & purification
  • Bacterial Proteins*
  • Cell Division / drug effects
  • Humans
  • In Vitro Techniques
  • Iron / metabolism*
  • Iron / pharmacology
  • Iron-Binding Proteins
  • Kinetics
  • Molecular Weight
  • Periplasmic Binding Proteins
  • Siderophores / biosynthesis*
  • Staphylococcus aureus / drug effects
  • Staphylococcus aureus / growth & development
  • Staphylococcus aureus / metabolism*

Substances

  • Bacterial Outer Membrane Proteins
  • Bacterial Proteins
  • Iron-Binding Proteins
  • Periplasmic Binding Proteins
  • Siderophores
  • Iron