Abstract
Growth rates, siderophore secretion, and bacterial proteins of two clinical isolates of Staphylococcus aureus were studied over 72 h of growth in iron-supplemented and iron-restricted chemically defined media. Under iron restriction the growth rates were decreased to different extents depending on the strain. Production of siderophore was detected in the mid-exponential and stationary phases of growth. The expression of iron-regulated proteins of 81, 23, and 17 kDa was time-dependent, associated with the same stage of growth, and might be involved in siderophore efficiency.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Outer Membrane Proteins / biosynthesis*
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Bacterial Outer Membrane Proteins / chemistry
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Bacterial Outer Membrane Proteins / isolation & purification
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Bacterial Proteins*
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Cell Division / drug effects
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Humans
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In Vitro Techniques
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Iron / metabolism*
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Iron / pharmacology
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Iron-Binding Proteins
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Kinetics
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Molecular Weight
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Periplasmic Binding Proteins
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Siderophores / biosynthesis*
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Staphylococcus aureus / drug effects
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Staphylococcus aureus / growth & development
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Staphylococcus aureus / metabolism*
Substances
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Bacterial Outer Membrane Proteins
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Bacterial Proteins
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Iron-Binding Proteins
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Periplasmic Binding Proteins
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Siderophores
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Iron