Abstract
We have isolated and sequenced a novel cDNA clone (D320), from methotrexate-selected multidrug-resistant (MDR) human leukemic CCRF-CEM cells, which encodes a peptide reactive with monoclonal antibody (mAb) C219. Despite having been cloned using this ostensibly P-glycoprotein-specific mAb, clone D320 has no significant homology with either the MDR-encoding gene or, at the amino acid (aa) level, with its product, P-glycoprotein. A putative C219-binding site has been identified in D320, which differs at two positions from the 6-aa C219 epitope previously described in P-glycoprotein.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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ATP Binding Cassette Transporter, Subfamily B, Member 1 / genetics
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ATP Binding Cassette Transporter, Subfamily B, Member 1 / immunology*
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Amino Acid Sequence
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Antibodies, Monoclonal
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Antibody Specificity
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Cross Reactions
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DNA, Complementary / genetics
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Drug Resistance, Multiple / genetics*
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Drug Resistance, Multiple / immunology
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Humans
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Leukemia / genetics*
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Methotrexate / pharmacology
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Molecular Sequence Data
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Neoplasm Proteins / genetics*
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Neoplasm Proteins / immunology*
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Recombinant Fusion Proteins / immunology
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Selection, Genetic
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Sequence Homology, Amino Acid
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Tumor Cells, Cultured
Substances
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ATP Binding Cassette Transporter, Subfamily B, Member 1
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Antibodies, Monoclonal
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DNA, Complementary
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Neoplasm Proteins
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Recombinant Fusion Proteins
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Methotrexate