An approach to human papillomavirus identification using low stringency single specific primer PCR

Mol Cell Probes. 1995 Feb;9(1):45-8. doi: 10.1016/s0890-8508(95)90992-3.

Abstract

A new PCR-based technique, low stringency single specific primer-PCR (LSSP-PCR), has been adapted to produce gene signatures capable of distinguishing human papillomaviruses (HPVs) in clinical specimens. This approach potentially offers a sensitive, inexpensive and high-throughput methodology for precise clinical diagnosis of HPV infections.

MeSH terms

  • Base Sequence
  • Consensus Sequence
  • DNA Primers*
  • Genes, Viral
  • Humans
  • Molecular Sequence Data
  • Papillomaviridae / genetics
  • Papillomaviridae / isolation & purification*
  • Papillomavirus Infections / diagnosis*
  • Polymerase Chain Reaction / methods*
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Tumor Virus Infections / diagnosis*

Substances

  • DNA Primers