Abstract
The acute effects of insulin on the activity of glycogen synthase kinase 3 (GSK-3) have been investigated both in the rat L6 muscle cell line and in cultured human myoblasts. The alpha and beta isoforms of GSK-3 are present in both cell types, with the beta isoform being predominant in the human cells. Insulin causes a rapid inactivation of both isoforms in both cell lines, with 50% inactivation being observed in the human myoblasts.
MeSH terms
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Amino Acid Sequence
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Animals
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Calcium-Calmodulin-Dependent Protein Kinases / antagonists & inhibitors*
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Calcium-Calmodulin-Dependent Protein Kinases / isolation & purification
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Calcium-Calmodulin-Dependent Protein Kinases / metabolism
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Cells, Cultured
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Chromatography, Gel
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Cyclic AMP-Dependent Protein Kinases / metabolism
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Glycogen Synthase Kinase 3
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Glycogen Synthase Kinases
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Humans
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Immunoblotting
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Insulin / pharmacology*
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Isoenzymes / antagonists & inhibitors*
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Isoenzymes / isolation & purification
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Isoenzymes / metabolism
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Kinetics
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Molecular Sequence Data
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Muscle, Skeletal / enzymology*
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Peptides / chemical synthesis
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Peptides / chemistry
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Phosphorylation
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Rats
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Substrate Specificity
Substances
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Insulin
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Isoenzymes
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Peptides
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Glycogen Synthase Kinases
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Cyclic AMP-Dependent Protein Kinases
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Calcium-Calmodulin-Dependent Protein Kinases
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Glycogen Synthase Kinase 3