Listeria monocytogenes isolation from human faecal specimens: experiments with the selective media, PALCAM and L-PALCAMY

Lett Appl Microbiol. 1993 Jan;16(1):32-5. doi: 10.1111/j.1472-765x.1993.tb01366.x.

Abstract

The selective media PALCAM and L-PALCAMY were evaluated for their potential ability to detect Listeria monocytogenes in faeces. Recovery on PALCAM was almost total, and similar at 30 degrees C and 35 degrees C with or without CO2 incubation. Warm enrichment in L-PALCAMY was necessary in order to detect low numbers (< 10(2)/ml faeces). Faeces in excess of 0.25 ml/10 ml L-PALCAMY was inhibitory. The results point to L-PALCAMY and PALCAM as an epidemiological tool.

MeSH terms

  • Culture Media*
  • Evaluation Studies as Topic
  • Feces / microbiology*
  • Humans
  • Listeria monocytogenes / growth & development
  • Listeria monocytogenes / isolation & purification*
  • Listeriosis / microbiology

Substances

  • Culture Media