The alpha 6 beta 1 integrin is a laminin receptor for developing retinal neurons

Cytotechnology. 1993:11 Suppl 1:S41-3.

Abstract

Cultured embryionic day 6 (E6) retinal neurons respond to laminin by making use of integrin receptors. We have recently shown that the laminin binding integrin receptor alpha 6 beta 1 is expressed in the chick retina on both retinal ganglion cells and other retinal neurons. Antibodies raised against a fusion protein containing a large fragment of the extracellular portion of the chick alpha 6 integrin subunit dramatically inhibit the interactions between E6 retinal neurons and laminin. These data show that alpha 6 beta 1 functions as a laminin receptor in these cells. In previous work we have also shown that the levels of the mRNA for alpha 6 decreases dramatically in retinal ganglion cells between E6 and E12. Data presented in this paper show that the decrease in alpha 6 mRNA is not prevented by ablation of the optic tectum, indicating that contact with the target is not a major cause for this decrease.

MeSH terms

  • Animals
  • Biotechnology
  • Cell Adhesion / physiology
  • Chick Embryo
  • Integrin alpha6beta1
  • Integrins / physiology*
  • Neurons / cytology
  • Neurons / physiology
  • RNA, Messenger / metabolism
  • Receptors, Laminin / physiology*
  • Retina / cytology
  • Retina / embryology
  • Retina / physiology*
  • Retinal Ganglion Cells / cytology
  • Retinal Ganglion Cells / physiology

Substances

  • Integrin alpha6beta1
  • Integrins
  • RNA, Messenger
  • Receptors, Laminin