Abstract
We have isolated human antibody fragments with binding specificities against the blood group antigens of the ABO and I blood group systems (B and HI), of the Rh system (D and E) and of the Kell system (Kpb), by selecting a phage-antibody library on human red cells. The fragments, expressed in bacteria, were antigen-specific and effective in assays including agglutination and immunohistochemistry. Selection of phage-antibody libraries using intact cells seems to offer a promising alternative to hybridoma technology for the production of antibodies against cell surface molecules.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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ABO Blood-Group System / immunology
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Amino Acid Sequence
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Antibody Specificity
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Antigens / immunology
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Blood Group Antigens / immunology*
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Humans
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I Blood-Group System / immunology
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Immunoglobulin Fragments / chemistry
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Immunoglobulin Fragments / immunology*
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Immunoglobulin Variable Region / chemistry
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Immunoglobulin Variable Region / immunology
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Kell Blood-Group System / immunology
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Molecular Sequence Data
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Rh-Hr Blood-Group System / immunology
Substances
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ABO Blood-Group System
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Antigens
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Blood Group Antigens
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I Blood-Group System
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Immunoglobulin Fragments
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Immunoglobulin Variable Region
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Kell Blood-Group System
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Rh-Hr Blood-Group System
Associated data
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GENBANK/Z23026
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GENBANK/Z23027
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GENBANK/Z23028
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GENBANK/Z23029
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GENBANK/Z23030
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GENBANK/Z23031
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GENBANK/Z23032
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GENBANK/Z23033
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GENBANK/Z23034
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GENBANK/Z23035
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GENBANK/Z23036
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GENBANK/Z25440