Abstract
Aminoglycoside resistance was investigated in six clinical isolates of Stenotrophomonas (Xanthomonas) maltophilia by studying the uptake kinetics and by using a radiochemical method to detect aminoglycoside modifying enzymes. Furthermore, the lipopolysaccharides (LPS) were extracted and characterized by SDS-PAGE and chemical analysis. Dansyl-polymyxin displacement experiments confirmed the availability of anionic binding sites. Growing cells of the isolates bound dansyl-polymyxin but were not lysed.
MeSH terms
-
Aminoglycosides
-
Anti-Bacterial Agents / metabolism*
-
Anti-Bacterial Agents / pharmacology
-
Bacterial Outer Membrane Proteins / metabolism
-
Binding Sites
-
Drug Resistance, Microbial
-
Electrophoresis, Polyacrylamide Gel
-
Lipopolysaccharides / chemistry
-
Lipopolysaccharides / metabolism*
-
Polymyxins / pharmacology
-
Xanthomonas / drug effects
-
Xanthomonas / enzymology
-
Xanthomonas / metabolism*
Substances
-
Aminoglycosides
-
Anti-Bacterial Agents
-
Bacterial Outer Membrane Proteins
-
Lipopolysaccharides
-
Polymyxins