Abstract
Crystals have been obtained of a ternary complex containing the yeast a1/alpha 2 homeodomain heterodimer bound to a 21-base pair DNA site containing two 5' overhanging bases at each end. The crystals are grown from cobaltic hexamine and form in space group P6(1) or P6(5) with a = b = 133 A, c = 45.4 A. Crystals that are flash-frozen at -179 degrees C diffract to 2.7 A along the c-axis and to 2.4 A in perpendicular directions. The crystals contain one protein-DNA complex in the crystallographic asymmetric unit.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Base Sequence
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Crystallization
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Crystallography, X-Ray
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DNA, Fungal / genetics
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DNA, Fungal / isolation & purification*
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DNA, Fungal / metabolism
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DNA-Binding Proteins / chemistry
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / isolation & purification*
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Fungal Proteins / chemistry
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Fungal Proteins / genetics
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Fungal Proteins / isolation & purification*
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Homeodomain Proteins*
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Minor Histocompatibility Antigens
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Molecular Sequence Data
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Proto-Oncogene Proteins c-bcl-2*
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Replication Protein C
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Repressor Proteins / chemistry
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Repressor Proteins / genetics
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Repressor Proteins / isolation & purification*
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Saccharomyces cerevisiae / chemistry
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae Proteins*
Substances
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BCL2-related protein A1
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DNA, Fungal
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DNA-Binding Proteins
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Fungal Proteins
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Homeodomain Proteins
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MATA1 protein, S cerevisiae
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MATA2 protein, S cerevisiae
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Minor Histocompatibility Antigens
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Proto-Oncogene Proteins c-bcl-2
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Repressor Proteins
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Saccharomyces cerevisiae Proteins
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Replication Protein C