Fluorometric assay of GTPase activity: application to the couple elongation factor eEF-2-ribosome

Anal Biochem. 1995 Feb 10;225(1):178-80. doi: 10.1006/abio.1995.1133.

Authors

P Gonzalo¹, B Sontag, D Guillot, J P Reboud

Affiliation

¹ Laboratoire de Biochimie Médicale, UPR CNRS 412, Lyon, France.

PMID: 7778776
DOI: 10.1006/abio.1995.1133

No abstract available

Publication types

Comparative Study

MeSH terms

GTP Phosphohydrolase-Linked Elongation Factors / analysis*
GTP Phosphohydrolase-Linked Elongation Factors / metabolism*
Guanosine Triphosphate / metabolism
Kinetics
Peptide Elongation Factor 2
Peptide Elongation Factors / metabolism*
Phosphorus Radioisotopes
Radioisotope Dilution Technique
Ribosomes / metabolism*
Sensitivity and Specificity
Spectrometry, Fluorescence / methods
Time Factors

Substances

Peptide Elongation Factor 2
Peptide Elongation Factors
Phosphorus Radioisotopes
Guanosine Triphosphate
GTP Phosphohydrolase-Linked Elongation Factors