Human interleukin for DA cells/leukemia inhibitory factor and oncostatin M enhance membrane expression of intercellular adhesion molecule-1 on melanoma cells but not the shedding of its soluble form

Cytokine. 1995 Feb;7(2):111-7. doi: 10.1006/cyto.1995.1015.

Abstract

ICAM-1-mediated cell-cell adhesion is essential for different immunologic functions including non-MHC-restricted cytotoxicity. The shedding of a soluble form of ICAM-1 from melanoma impairs immune recognition and leads to tumour escape. Pretreatment of the Foss human melanoma cell line with HILDA/LIF or OSM, two cytokines involved in acute-phase response, increased the expression of membrane ICAM-1 twofold without inducing sICAM-1 shedding. Conversely, TNF-alpha, in the same conditions, strongly stimulated membrane ICAM-1 expression and the shedding of the soluble form. The same phenomenon was observed on the A375 human melanoma cell line. ICAM-1 upregulation was concomitant with an increase in the non-MHC-restricted cytotoxicity of tumour cells mediated by LAK cell.s This higher sensitivity to LAK lysis was abolished by RR1/1, a specific monoclonal antibody against ICAM-1. Our results demonstrate for the first time the ability of HILDA/LIF and OSM to upregulate ICAM-1 expression on the melanoma cell surface, suggesting a potential role for these cytokines in human immune surveillance during tumour progression.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Binding Sites
  • Cell Line
  • Cell Membrane / metabolism*
  • Cell Survival / drug effects
  • Flow Cytometry
  • Gene Expression / drug effects*
  • Gene Expression Regulation, Neoplastic / drug effects
  • Growth Inhibitors / pharmacology*
  • Humans
  • Intercellular Adhesion Molecule-1 / biosynthesis*
  • Intercellular Adhesion Molecule-1 / metabolism
  • Interleukin-6*
  • Kinetics
  • Leukemia Inhibitory Factor
  • Lymphokines / pharmacology*
  • Melanoma
  • Oncostatin M
  • Peptides / pharmacology*
  • Recombinant Proteins / pharmacology
  • Time Factors
  • Tumor Cells, Cultured
  • Tumor Necrosis Factor-alpha / pharmacology

Substances

  • Growth Inhibitors
  • Interleukin-6
  • LIF protein, human
  • Leukemia Inhibitory Factor
  • Lymphokines
  • OSM protein, human
  • Peptides
  • Recombinant Proteins
  • Tumor Necrosis Factor-alpha
  • Oncostatin M
  • Intercellular Adhesion Molecule-1