Abstract
In order to determine the genomic heterogeneity of ovine lentiviruses, we analysed eight isolates from naturally infected sheep from one geographical region of France. A 475 nt fragment in the region of the pol gene coding for reverse transcriptase was amplified by RT-PCR from RNA directly extracted from uncultured bronchoalveolar lavage cells. The resulting PCR fragments were analysed by restriction enzyme digestion, cloned in a TA vector and sequenced. Restriction enzyme analysis showed distinct patterns from the eight isolates, and sequencing showed them to be closely related in both nucleotide (2.3-8.1% variation) and deduced amino acid (0-6.2% variation) sequences. Their amino acid sequences differed from that of visna-maedi virus complete viral genome sequence K1514 by 12.5-15.3%, but from that of caprine arthritis encephalitis virus (CAEV) viral genome sequence Co by only 4.2-6.9%. Phylogenetic analysis showed that the French isolates form a group related to CAEV Co and distant from previously reported ovine lentivirus sequences from different origins.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Arthritis-Encephalitis Virus, Caprine / genetics*
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Base Sequence
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Bronchoalveolar Lavage Fluid / cytology
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Bronchoalveolar Lavage Fluid / virology*
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France
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Gene Products, pol / genetics
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Genes, pol*
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Genetic Variation
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Geography
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Lentiviruses, Ovine-Caprine / genetics*
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Lentiviruses, Ovine-Caprine / isolation & purification
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Molecular Sequence Data
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Phylogeny*
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Polymerase Chain Reaction
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RNA, Viral / genetics
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RNA, Viral / isolation & purification
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Restriction Mapping
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Sequence Homology, Amino Acid
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Sequence Homology, Nucleic Acid
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Sheep / virology*
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Visna-maedi virus / genetics*
Substances
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Gene Products, pol
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RNA, Viral
Associated data
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GENBANK/U35674
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GENBANK/U35675
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GENBANK/U35676
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GENBANK/U35677
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GENBANK/U35678
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GENBANK/U35679
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GENBANK/U35680
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GENBANK/U35681
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GENBANK/U35795
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GENBANK/U35796
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GENBANK/U35797
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GENBANK/U35798
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GENBANK/U35799
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GENBANK/U35800
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GENBANK/U35801
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GENBANK/U35802
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GENBANK/U35803
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GENBANK/U35804
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GENBANK/U35805
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GENBANK/U35806
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GENBANK/U35807
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GENBANK/U35808
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GENBANK/U35809
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GENBANK/U35810
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GENBANK/U35811
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GENBANK/U35812
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GENBANK/U35813
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GENBANK/U35814
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GENBANK/U35857
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GENBANK/U35858