Parasitic culture of buffy coat for diagnosis of visceral leishmaniasis in human immunodeficiency virus-infected patients

R López-Vélez; F Laguna; J Alvar; J A Pérez-Molina; R Molina; P Martinez; J Villarrubia

doi:10.1128/jcm.33.4.937-939.1995

Parasitic culture of buffy coat for diagnosis of visceral leishmaniasis in human immunodeficiency virus-infected patients

J Clin Microbiol. 1995 Apr;33(4):937-9. doi: 10.1128/jcm.33.4.937-939.1995.

Authors

R López-Vélez¹, F Laguna, J Alvar, J A Pérez-Molina, R Molina, P Martinez, J Villarrubia

Affiliation

¹ Tropical Medicine and Clinical Parasitology Unit, Hospital Ramón y Cajal, Madrid, Spain.

Abstract

Two samples of buffy coat from the peripheral blood of 25 human immunodeficiency virus-positive patients with proven visceral leishmaniasis, as determined with a bone marrow aspirate (stain and culture), were cultured onto Schneider's and Novy-McNeal-Nicolle media. Hemoculture positivity was 67%. The average growing time was 10 days. This is an easy, noninvasive, and sensitive technique.

Publication types

Clinical Trial

MeSH terms

AIDS-Related Opportunistic Infections / complications*
AIDS-Related Opportunistic Infections / diagnosis*
AIDS-Related Opportunistic Infections / parasitology
Animals
Bone Marrow / parasitology
Female
Humans
Leishmania / isolation & purification*
Leishmaniasis, Visceral / complications*
Leishmaniasis, Visceral / diagnosis*
Leishmaniasis, Visceral / parasitology
Male
Parasitemia / complications*
Parasitemia / diagnosis*
Parasitemia / parasitology
Parasitology / methods*
Parasitology / statistics & numerical data
Phlebotomus
Sensitivity and Specificity