Straying phenomenon of migrating LHRH neurons and highly polysialylated NCAM in the chick embryo

Neurosci Res. 1995 Mar;22(1):109-15. doi: 10.1016/0168-0102(95)00883-u.

Abstract

The present study on unilaterally incompletely placodectomized chick embryos revealed that arrival of migrating LHRH neurons into the forebrain area is dependent on the presence of a central projection of the olfactory nerve. When a fragment of the lateral and medial olfactory epithelium was spared the damage, a small number of LHRH-ir cells were found to migrate into the forebrain along a thin NCAM-H-expressing fiber bundle of the olfactory nerve. In embryos with large lesions which destroyed the lateral olfactory epithelium, the poorly developed NCAM-H-positive olfactory nerve fibers were arrested where they meet the NCAM-positive medial nasal branch of the ophthalmic nerve and frequently fused with this branch fiber bundle. In these embryos, no LHRH-ir cells were detected in the forebrain area. They were deviated from their regular migratory course to the NCAM-H-positive medial nasal branch fiber bundle. This straying phenomenon indicates that some structural support is apparently needed for the migration of LHRH-ir cells, but their migratory route is not completely programmed in their precursor cells in the olfactory placode. In cultures of placodal tissues, the coexistence of migrating LHRH-ir cells with NCAM-H-expressing neural elements was always confirmed. It is suggested therefore that not only the structural support for the migration but also the interaction between LHRH-ir cells and NCAM-H-expressing neural elements is a prerequisite for successful LHRH neuronal migration.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Adhesion Molecules, Neuronal / metabolism*
  • Cells, Cultured
  • Chick Embryo
  • Electrodes, Implanted
  • Gonadotropin-Releasing Hormone / metabolism*
  • Immunohistochemistry
  • Nerve Endings / metabolism
  • Neural Pathways / cytology
  • Neural Pathways / embryology
  • Neural Pathways / metabolism
  • Neurons / metabolism*
  • Neurons / physiology
  • Olfactory Nerve / cytology
  • Olfactory Nerve / embryology
  • Olfactory Nerve / metabolism
  • Sialic Acids / metabolism*

Substances

  • Cell Adhesion Molecules, Neuronal
  • Sialic Acids
  • Gonadotropin-Releasing Hormone