Use of cytosol-depleted HL-60 cells for reconstitution studies of G-protein-regulated phosphoinositide-specific phospholipase C-beta isozymes

Methods Enzymol. 1994:238:154-68. doi: 10.1016/0076-6879(94)38014-7.
No abstract available

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins
  • Cell Line
  • Cell Membrane Permeability
  • Cytosol / physiology
  • Electrophoresis, Polyacrylamide Gel
  • GTP-Binding Proteins / physiology*
  • Guanosine 5'-O-(3-Thiotriphosphate) / pharmacology
  • Homeostasis
  • Humans
  • Indicators and Reagents
  • Isoenzymes / isolation & purification
  • Isoenzymes / metabolism*
  • Kinetics
  • Leukemia, Promyelocytic, Acute
  • Phosphatidylinositol Diacylglycerol-Lyase
  • Phosphoric Diester Hydrolases / isolation & purification
  • Phosphoric Diester Hydrolases / metabolism*
  • Radioisotope Dilution Technique
  • Streptolysins
  • Tritium
  • Tumor Cells, Cultured

Substances

  • Bacterial Proteins
  • Indicators and Reagents
  • Isoenzymes
  • Streptolysins
  • streptolysin O
  • Tritium
  • Guanosine 5'-O-(3-Thiotriphosphate)
  • Phosphoric Diester Hydrolases
  • GTP-Binding Proteins
  • Phosphatidylinositol Diacylglycerol-Lyase