Autoregulation of endothelin-1 secretion by cultured human keratinocytes via the endothelin B receptor

Biochim Biophys Acta. 1994 Dec 30;1224(3):454-8. doi: 10.1016/0167-4889(94)90282-8.

Abstract

We investigated endothelin-1 (ET-1) receptor expression on normal human keratinocytes (HK). We show that HK express the ETB receptor isoform and respond to ET-1 with a 2.7-fold increase in intracellular free calcium. HK did not respond to ET-1 with increased proliferation; however, 30 nM ET-1 caused a 51.7% decrease in ET-1 accumulation in HK-conditioned medium. We propose that HK ET-1 receptors function in autocrine regulation of ET-1 secretion.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cell Division
  • Cells, Cultured
  • Culture Media, Conditioned
  • Endothelins / metabolism*
  • Humans
  • Keratinocytes / cytology
  • Keratinocytes / metabolism*
  • Protein Binding
  • Receptor, Endothelin B
  • Receptors, Endothelin / physiology*

Substances

  • Culture Media, Conditioned
  • Endothelins
  • Receptor, Endothelin B
  • Receptors, Endothelin