A 14.4 KDa acidic subunit of human RNA polymerase II with a putative leucine-zipper

DNA Seq. 1994;4(5):329-31. doi: 10.3109/10425179409020860.

Abstract

The cDNA of a small subunit (hRPB14.4) of RNA polymerase II (or B) from HeLa cells has been cloned. A 127 residue peptide sequence (calculated molecular weight of 14,478; isoelectric point of 3.7) was deduced and compared to that of the homologous subunit of Saccharomyces cerevisiae polymerase (ABC23, encoded by the RPB6/RPO26 gene). About 50% of the total residues were found to be conserved between yeast and man, with the C-terminal two-third being the most conserved (72% identity). A putative leucine-zipper comprising four properly spaced leucine residues, but not preceded by a basic domain, was identified near the C-terminal end of both proteins.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • DNA
  • DNA, Complementary
  • HeLa Cells
  • Humans
  • Hydrogen-Ion Concentration
  • Leucine Zippers*
  • Molecular Sequence Data
  • RNA Polymerase II / chemistry
  • RNA Polymerase II / genetics*
  • Saccharomyces cerevisiae / enzymology
  • Sequence Homology, Amino Acid

Substances

  • DNA, Complementary
  • DNA
  • RNA Polymerase II

Associated data

  • GENBANK/Z27113