Measurement of antioxidants in human blood plasma

Methods Enzymol. 1994:234:269-79. doi: 10.1016/0076-6879(94)34094-3.

Abstract

The concentration of antioxidants in human blood plasma is important in investigating and understanding the relationship between diet, oxidant stress, and human disease. The HPLC-EC technique combines selectivity with high sensitivity for measuring both water- and lipid-soluble antioxidants. The excellent sensitivity of the methods described here allows one to measure a panel of antioxidants in a small volume of plasma.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antioxidants / analysis*
  • Ascorbic Acid / blood*
  • Bilirubin / blood
  • Blood Chemical Analysis / methods*
  • Blood Proteins / analysis
  • Carotenoids / blood
  • Chromatography, High Pressure Liquid / methods
  • Diet
  • Disease
  • Electrochemistry / methods
  • Humans
  • Indicators and Reagents
  • Lycopene
  • Sulfhydryl Compounds / blood*
  • Ubiquinone / analogs & derivatives
  • Ubiquinone / blood
  • Uric Acid / blood
  • Vitamin E / blood
  • beta Carotene

Substances

  • Antioxidants
  • Blood Proteins
  • Indicators and Reagents
  • Sulfhydryl Compounds
  • beta Carotene
  • Ubiquinone
  • Vitamin E
  • Uric Acid
  • Carotenoids
  • ubiquinol
  • Ascorbic Acid
  • Bilirubin
  • Lycopene