Alterations of binding mode and cutting site by G-->I replacement in preferred cleavage sequences 5'-AGG of chromoprotein C-1027

Biochem Biophys Res Commun. 1994 Dec 30;205(3):1533-8. doi: 10.1006/bbrc.1994.2841.

Abstract

Replacement of guanosine by inosine at the center position of a target trinucleotide 5'-AGG resulted in greatly diminished DNA cleavage by C-1027 chromophore, indicating that the guanine 2-amino group significantly participates to DNA binding and cleavage by the chromophore of C-1027. This notion was supported by fluorescence titrations of the chromophore with [poly(dG-dC)]2 and [poly(dI-dC)]2. In preferred cleavage sequence 5'-AGG of the enediyne chromophore of C-1027, the G-->I substitution clearly induces change of DNA binding mode and cutting site.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aminoglycosides*
  • Anti-Bacterial Agents* / chemistry
  • Anti-Bacterial Agents* / metabolism*
  • Anti-Bacterial Agents* / pharmacology
  • Base Sequence
  • Binding Sites / genetics
  • DNA / drug effects
  • DNA / genetics
  • DNA / metabolism*
  • Enediynes
  • In Vitro Techniques
  • Molecular Sequence Data
  • Oligodeoxyribonucleotides / genetics
  • Oligodeoxyribonucleotides / metabolism
  • Peptides*
  • Poly dA-dT / metabolism
  • Polydeoxyribonucleotides / metabolism
  • Spectrometry, Fluorescence

Substances

  • Aminoglycosides
  • Anti-Bacterial Agents
  • Enediynes
  • Oligodeoxyribonucleotides
  • Peptides
  • Polydeoxyribonucleotides
  • C 1027
  • poly(dG).poly(dC)
  • poly d(I-C)
  • Poly dA-dT
  • DNA