Resonance assignment of methionine methyl groups and chi 3 angular information from long-range proton-carbon and carbon-carbon J correlation in a calmodulin-peptide complex

J Biomol NMR. 1994 Nov;4(6):787-97. doi: 10.1007/BF00398409.

Abstract

Several simple 3D experiments are used to provide J correlations between methionine C epsilon methyl carbons and either the C gamma H2 protons or C beta and C gamma. The intensity of the J correlations provides information on the size of the three-bond J couplings and thereby on the chi 3 torsion angle. In addition, a simple 3D version of the HMBC experiment provides a sensitive link between the C epsilon H3 methyl protons and C gamma. The methods are demonstrated for a 20 kDa complex between calmodulin and a 26-residue peptide fragment of skeletal muscle myosin light chain kinase.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Calmodulin / chemistry*
  • Calmodulin-Binding Proteins / chemistry
  • Carbon
  • Magnetic Resonance Spectroscopy
  • Methionine / chemistry*
  • Molecular Sequence Data
  • Myosin-Light-Chain Kinase / chemistry*
  • Peptides / chemistry
  • Protons

Substances

  • Calmodulin
  • Calmodulin-Binding Proteins
  • Peptides
  • Protons
  • Carbon
  • Methionine
  • Myosin-Light-Chain Kinase