Abstract
A 29 kDa protein, isolated from the outer membrane of Coxiella burnetii, strain Nine Mile phase I by detergent Empigen BB, was characterized. The failure in removing lipopolysaccharides (LPS) from preparations of the protein by the purification method used indicates a strong binding between proteins and LPS in the outer membrane of C. burnetii. The protein was immunogenic in mice and protected them against virulent C. burnetii challenge.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibody Formation
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Bacterial Outer Membrane Proteins / analysis*
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Bacterial Outer Membrane Proteins / physiology
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Blotting, Western
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Coxiella burnetii / chemistry*
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Coxiella burnetii / classification
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Coxiella burnetii / immunology
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Coxiella burnetii / pathogenicity
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Detergents*
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Disease Models, Animal
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Electrophoresis, Gel, Two-Dimensional
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Lipopolysaccharides / metabolism
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Mice
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Mice, Inbred BALB C
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Organic Chemicals
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Protein Binding
Substances
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Bacterial Outer Membrane Proteins
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Detergents
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Lipopolysaccharides
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Organic Chemicals
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empigen BB