Use of the primed in situ labelling (PRINS) technique for a rapid detection of chromosomes 13, 16, 18, 21, X and Y

F Pellestor; A Girardet; G Lefort; B Andréo; J P Charlieu

doi:10.1007/BF00225066

Use of the primed in situ labelling (PRINS) technique for a rapid detection of chromosomes 13, 16, 18, 21, X and Y

Hum Genet. 1995 Jan;95(1):12-7. doi: 10.1007/BF00225066.

Authors

F Pellestor¹, A Girardet, G Lefort, B Andréo, J P Charlieu

Affiliation

¹ CNRS UPR 9008, Montpellier, France.

PMID: 7814017
DOI: 10.1007/BF00225066

Abstract

The primed in situ labelling (PRINS) technique is an alternative to in situ hybridization for chromosomal screening. We have developed a semi-automatic PRINS protocol, using a programmable thermocycler. The method has been successfully tested with specific primers for chromosomes, 13, 16, 18, 21, X and Y. Specific chromosome detection has been obtained on both metaphases and interphase nuclei. This suggests that PRINS may be a reliable technique for detecting aneuploidies and some chromosomal aberrations.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
Chromosomes, Human*
Chromosomes, Human, Pair 13
Chromosomes, Human, Pair 16
Chromosomes, Human, Pair 18
Chromosomes, Human, Pair 21
Cytogenetics / methods*
DNA Primers
Humans
Molecular Sequence Data
Nucleic Acid Hybridization*
X Chromosome
Y Chromosome

Substances

DNA Primers