The effects of a football match on the muscle metabolism of long-term football players were studied. Twenty-two senior high school students (Age: 16.95 +/- 0.82 y/o) were divided into two groups. Group A, included the subjects with longer histories of playing football (7 years) and group B were those with a shorter histories (6 years). There was no statistical difference between the two groups (p > 0.05). The temperature of all muscles tested showed statistical differences after exercise (p < 0.05). After 24 hours, all muscles except the gluteus maximus returned to the normal state while the skin temperature of the latissimus dorsi did not have any change. Body temperature, pulse rate, respiratory rate, and diastolic pressure all significantly increased immediately after the match (p < 0.05) and they all returned to their normal resting rate within one hour, except the body temperature, which required two hours to return to normal. A comparison between groups A and B showed a significant difference (p < 0.01) in creatine kinase level immediately and one hour after the match. It is inferred that energy is derived from the aerobic system.