Stimulatory effect of angiotensin II on calcium efflux from cultured bovine adrenal chromaffin cells

Life Sci. 1995;56(5):PL109-14. doi: 10.1016/0024-3205(94)00962-7.

Abstract

The effect of angiotensin II on Ca2+ mobilization in cultured bovine adrenal chromaffin cells was examined. Angiotensin II (10(-7)M) increased the intracellular free Ca2+ level ([Ca2+]i) to a peak in the presence or absence of extracellular Ca2+, followed by decrease with time. Angiotensin II (10(-9)-10(-6)M) also stimulated 45Ca2+ efflux from cultured bovine adrenal chromaffin cells in a concentration-dependent manner. Its stimulatory effect on 45Ca2+ efflux was inhibited by the angiotensin II antagonist [Sar1, Ile8]-angiotensin II or [Sar1, Val5, Ala8]-angiotensin II. The increase in angiotensin II-stimulated 45Ca2+ efflux was dependent on the extracellular Na+ concentration. Angiotensin II also increased 22Na+ influx into the cells. These results indicate that stimulation of the angiotensin II receptor induces extracellular Na(+)-dependent Ca2+ efflux from cultured bovine adrenal chromaffin cells, probably by acceleration of Na+/Ca2+ exchange.

MeSH terms

  • Adrenal Medulla / cytology
  • Adrenal Medulla / drug effects*
  • Adrenal Medulla / metabolism*
  • Angiotensin II / pharmacology*
  • Animals
  • Calcium / metabolism*
  • Calcium Radioisotopes
  • Carrier Proteins / drug effects
  • Carrier Proteins / metabolism
  • Cattle
  • Cells, Cultured
  • Chromaffin System / cytology
  • Chromaffin System / drug effects*
  • Chromaffin System / metabolism*
  • Extracellular Space / metabolism
  • Sodium / metabolism
  • Sodium Radioisotopes
  • Sodium-Calcium Exchanger
  • Stimulation, Chemical

Substances

  • Calcium Radioisotopes
  • Carrier Proteins
  • Sodium Radioisotopes
  • Sodium-Calcium Exchanger
  • Angiotensin II
  • Sodium
  • Calcium