Three-dimensional reconstruction of mammalian 40 S ribosomal subunit embedded in ice

J Mol Biol. 1995 Feb 3;245(5):461-6. doi: 10.1006/jmbi.1994.0037.

Abstract

A platform-like structure, which appears equivalent to the platform or lobe structure of the 30 S subunit of the eubacterial ribosome, is observed in the reconstruction of the small 40 S ribosomal subunit from images of ice-embedded particles. This cup-shaped structure, 15.0 nm in side length and 13.5 nm wide at its rim, extends obliquely upward on the back of the subunit. Other previously characterized features of the 40 S subunit can readily be identified: the head with its prominent beak structure, the body with its two back lobes expressed as relatively small-scale features, and the two widely separated feet that comprise the base of the subunit.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Ice
  • Mammals
  • Microscopy, Electron / methods
  • Protein Conformation
  • Ribosomal Proteins / chemistry*
  • Ribosomal Proteins / ultrastructure

Substances

  • Ice
  • Ribosomal Proteins
  • Rpsa protein, rat