Abstract
In ten transgenic lines, expression of a human mid-sized (M) neurofilament (NF) transgene was restricted to neurons in the central and peripheral nervous systems. However, no two lines gave identical expression patterns and none exactly matched the expression of mouse NF(M). These varied expression patterns within the neural compartment likely result from interactions of the transgene with enhancer elements located in the regions flanking the insertion site. Unexpected patterns of enhancer activity included an enhancer active in subsets of cerebellar basket cells as well as others preferentially active in subsets of motor or sensory neurons.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Axons / metabolism
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Base Sequence
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Brain / metabolism*
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Crosses, Genetic
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Enhancer Elements, Genetic*
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Female
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Fluorescent Antibody Technique
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Humans
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Male
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Mice
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Mice, Inbred C57BL
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Mice, Inbred DBA
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Mice, Transgenic
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Molecular Sequence Data
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Neurofilament Proteins / analysis
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Neurofilament Proteins / biosynthesis*
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Neurofilament Proteins / genetics*
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Neurons / metabolism*
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Oligodeoxyribonucleotides
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Organ Specificity
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RNA, Messenger / analysis
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RNA, Messenger / biosynthesis
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Spinal Cord / metabolism*
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Trigeminal Nerve / metabolism
Substances
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Neurofilament Proteins
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Oligodeoxyribonucleotides
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RNA, Messenger