Transverse pore gradient gel electrophoresis, using the PhastSystem

Electrophoresis. 1994 Aug-Sep;15(8-9):1028-31. doi: 10.1002/elps.11501501153.

Abstract

The application of pore gradient gels prefabricated for the PhastSystem (Pharmacia) to transverse pore gradient gel electrophoresis is demonstrated. It has the twofold advantage of (i) horizontal positioning, avoiding gel stretching during the preparation of these gels and resulting pore size irreproducibility experienced with vertically applied pore gradient gels, which necessitate an orthogonal transfer of spacers, and (ii) miniaturized gel dimensions, which allow a small sample load and a short duration of electrophoresis and staining.

MeSH terms

  • Base Composition
  • DNA / chemistry
  • DNA / isolation & purification*
  • Electrophoresis, Polyacrylamide Gel / instrumentation
  • Electrophoresis, Polyacrylamide Gel / methods*
  • Indicators and Reagents
  • Molecular Weight
  • Software

Substances

  • Indicators and Reagents
  • DNA