Abstract
Infectious virus resembling type D simian retrovirus (SRV) was isolated from Ethiopian baboons (Papio cynocephalus) (SRV-Pc) housed at the University of Washington Regional Primate Research Center. When baboon peripheral blood mononuclear cells (PBMC) or tissues were cocultured with the H-9 human T-cell line or the Raji human B-cell line, large multinucleated syncytia positive for SRV-2 antigens were observed microscopically. Immunoblot analysis of purified SRV-Pc from cell culture supernatants demonstrated that the viral core and envelope proteins reacted with rabbit anti-SRV-2 serum. Fresh PBMC and cocultured cells were positive by polymerase chain reaction using two different sets of SRV-2 primers. Preliminary sequence analysis of two separate isolates from portions of the SRV-Pc p27 and gp20 regions revealed homology with SRV-1, SRV-2, and Mason-Pfizer monkey virus. The homologies in the p27 segment were 91-94% and the homologies in the gp20 segment were 72-75%.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Antibodies, Viral / blood
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Antigens, Viral / analysis
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Cross Reactions
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Female
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Fetal Death / veterinary
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Fetal Death / virology
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Gene Products, env / analysis
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Gene Products, env / genetics
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Gene Products, gag / analysis
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Gene Products, gag / genetics
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Male
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Molecular Sequence Data
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Monkey Diseases / virology*
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Papio*
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Retroviridae Infections / immunology
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Retroviridae Infections / veterinary*
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Retroviridae Infections / virology
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Retroviruses, Simian / genetics
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Retroviruses, Simian / immunology
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Retroviruses, Simian / isolation & purification*
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Sequence Alignment
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Sequence Analysis, DNA
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Sequence Homology, Amino Acid
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Sequence Homology, Nucleic Acid
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Tumor Virus Infections / immunology
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Tumor Virus Infections / veterinary*
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Tumor Virus Infections / virology
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Viral Envelope Proteins / analysis
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Viral Envelope Proteins / genetics
Substances
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Antibodies, Viral
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Antigens, Viral
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Gene Products, env
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Gene Products, gag
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Viral Envelope Proteins
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simian retrovirus type D envelope protein gp20
Associated data
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GENBANK/U16843
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GENBANK/U16844