Abstract
Characterization of Pisum (pea) seed trypsin inhibitors (TI) and their corresponding cDNAs indicates that the pea TI gene family contains two genes. The existence of multiple TI isoforms can be attributed to post-translational modifications of primary gene products. Post-translational processing at the C-terminus during the desiccation stage of seed development results in the appearance of TI isoforms with increased affinity for the target enzyme, trypsin.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Chromatography, Affinity
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DNA, Complementary
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Gene Expression Regulation, Developmental
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Genes, Plant
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Mass Spectrometry / methods
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Molecular Sequence Data
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Pisum sativum / chemistry
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Pisum sativum / genetics*
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Pisum sativum / metabolism
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Protein Processing, Post-Translational
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Trypsin Inhibitors / genetics*
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Trypsin Inhibitors / isolation & purification
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Trypsin Inhibitors / metabolism
Substances
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DNA, Complementary
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RNA, Messenger
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Trypsin Inhibitors
Associated data
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GENBANK/X83210
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GENBANK/X83211