Variable-region sequences for T-cell receptor-gamma and -delta chains of rabbit killer cell lines against Shope carcinoma cells

Cancer Lett. 1995 Feb 10;89(1):37-44. doi: 10.1016/0304-3835(95)90155-8.

Abstract

A rabbit gamma delta killer T-cell line against Shope carcinoma cells was established from peripheral blood lymphocytes (PBL) of a human T-lymphotropic virus type I (HTLV-I)-infected rabbit bearing Shope papilloma and carcinoma. Southern hybridization analysis of this cell line with an HTLV-I probe showed that the cell line contained multiple clones of HTLV-I-transformed cells, and three sublines with different integration patterns of the HTLV-1 genome were isolated by cloning of the cell line. In all these sublines T-cell receptor (TCR)-gamma and -delta genes were rearranged and expressed. A PCR-based analysis of the expression of variable (V) genes showed that the killer cell line preferentially expressed V gamma 1.1 and V delta 1 genes, whereas V gamma 2 and V delta 1 genes were dominantly expressed in normal PBL. Analysis of the junctional sequences of TCR-gamma and -delta genes which dictate the fine specificities of epitope recognition revealed that all three sublines expressed V gamma 1.1/V delta 1 genes without the nucleotide diversity at the V-J junctions.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Carcinoma, Squamous Cell / blood*
  • Carcinoma, Squamous Cell / virology*
  • Cell Transformation, Viral
  • Cottontail rabbit papillomavirus*
  • Gene Rearrangement, delta-Chain T-Cell Antigen Receptor / genetics
  • Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor / genetics
  • Genome, Viral
  • HTLV-I Infections / blood
  • HTLV-I Infections / virology
  • Human T-lymphotropic virus 1 / genetics
  • Humans
  • Immunoglobulin Variable Region / genetics*
  • Killer Cells, Natural / physiology*
  • Killer Cells, Natural / virology
  • Molecular Sequence Data
  • Papillomavirus Infections / blood*
  • Rabbits
  • Receptors, Antigen, T-Cell, gamma-delta / genetics*
  • Transcription, Genetic
  • Tumor Cells, Cultured
  • Tumor Virus Infections / blood*
  • Virus Integration

Substances

  • Immunoglobulin Variable Region
  • Receptors, Antigen, T-Cell, gamma-delta

Associated data

  • GENBANK/D26555
  • GENBANK/D38118
  • GENBANK/D38119
  • GENBANK/D38120
  • GENBANK/D38121
  • GENBANK/D38134
  • GENBANK/D38137
  • GENBANK/D38138
  • GENBANK/D38139
  • GENBANK/D38142