Isolation of a human cerebral imidazoline-specific binding protein

H Greney; F Bennai; A Molines; A Belcourt; M Dontenwill; P Bousquet

doi:10.1016/0014-2999(94)90234-8

Isolation of a human cerebral imidazoline-specific binding protein

Eur J Pharmacol. 1994 Nov 14;265(1-2):R1-2. doi: 10.1016/0014-2999(94)90234-8.

Authors

H Greney¹, F Bennai, A Molines, A Belcourt, M Dontenwill, P Bousquet

Affiliation

¹ Laboratoire de Pharmacologie Cardiovasculaire et Rénale, C.N.R.S. URA 589, Université Louis Pasteur, Faculté de Médecine, Strasbourg, France.

PMID: 7883019
DOI: 10.1016/0014-2999(94)90234-8

Abstract

The first isolation of a human brain specific imidazoline binding protein is described. This protein was obtained using affinity chromatography and was revealed with the aid of an anti-idiotypic antibody specific for imidazoline binding sites. The protein (43 kDa) differs from other imidazoline binding proteins previously isolated from peripheral tissues, in particular by being also sensitive to clonidine.

MeSH terms

Antibodies, Anti-Idiotypic / metabolism
Antibody Specificity
Carrier Proteins / drug effects
Carrier Proteins / isolation & purification*
Carrier Proteins / metabolism
Cerebral Cortex / metabolism*
Chromatography, Affinity
Clonidine / pharmacology
Electrophoresis, Polyacrylamide Gel
Humans
Imidazoles / metabolism*
Imidazoline Receptors
Immunoblotting
Molecular Weight
Receptors, Drug / metabolism*

Substances

Antibodies, Anti-Idiotypic
Carrier Proteins
Imidazoles
Imidazoline Receptors
Receptors, Drug
Clonidine