Abstract
We describe in this report a computer program for easy interpretation of polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) patterns generated during HLA Class II typing by the modified PCR-RFLP method. HLA-DQA1, HLA-DQB1, HLA-DRB1 and HLA-DPB1 typing result analysis are thus greatly simplified. The program also allows video capture and image storage, which are becoming a new standard in molecular biology laboratories.
MeSH terms
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Genotype*
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HLA Antigens / genetics*
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HLA-DP Antigens / genetics
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HLA-DP beta-Chains
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HLA-DQ Antigens / genetics
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HLA-DQ alpha-Chains
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HLA-DQ beta-Chains
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HLA-DR Antigens / genetics
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HLA-DRB1 Chains
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Histocompatibility Antigens Class II / genetics
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Humans
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Polymerase Chain Reaction*
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Polymorphism, Restriction Fragment Length*
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Software*
Substances
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HLA Antigens
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HLA-DP Antigens
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HLA-DP beta-Chains
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HLA-DPB1 antigen
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HLA-DQ Antigens
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HLA-DQ alpha-Chains
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HLA-DQ beta-Chains
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HLA-DQA1 antigen
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HLA-DQB1 antigen
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HLA-DR Antigens
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HLA-DRB1 Chains
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Histocompatibility Antigens Class II