Abstract
Two lines of transgenic mice were developed which differ in their level of expression of V beta 11. To determine the role of TCR density in tolerance induction, these mice were bred with I-E expressing mice and were investigated for tolerance induction. T cells expressing V beta 11 at high density are deleted by negative selection. T cells expressing < 10% of the normal TCR receptor density are not subject to negative selection and were not activated in vitro. There is a correlation in receptor density in both strains of mice with in vitro activation. These findings support the notion that there is a definable quantitative signal threshold which is critical for tolerance induction.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Antigens, Differentiation, T-Lymphocyte / biosynthesis
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CD2 Antigens
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CD4-Positive T-Lymphocytes / immunology
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Flow Cytometry
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Lymph Nodes / cytology
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Lymphocyte Activation
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Mice
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Mice, Transgenic / immunology*
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Receptors, Antigen, T-Cell, alpha-beta / genetics
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Receptors, Antigen, T-Cell, alpha-beta / metabolism
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Receptors, Antigen, T-Cell, alpha-beta / physiology*
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Receptors, Immunologic / biosynthesis
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Self Tolerance / genetics
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Self Tolerance / immunology*
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Spleen / cytology
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T-Lymphocytes / immunology*
Substances
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Antigens, Differentiation, T-Lymphocyte
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CD2 Antigens
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Receptors, Antigen, T-Cell, alpha-beta
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Receptors, Immunologic