Application of a recombinant baculovirus expressing hantavirus nucleocapsid protein as a diagnostic antigen in IFA test: cross reactivities among 3 serotypes of hantavirus which causes hemorrhagic fever with renal syndrome (HFRS)

J Vet Med Sci. 1993 Dec;55(6):1047-50. doi: 10.1292/jvms.55.1047.

Abstract

Recombinant baculovirus-infected insect cells which expressed recombinant protein, analogous to the nucleocapsid protein (NP) of Seoul type hantavirus, strain SR-11 (rNP-SR-Sf9) were applied to the indirect immunofluorescent antibody (IFA) test. The rNP-SR-Sf9 reacted with anti NP MAb clones which recognized strain specific or hantavirus common epitopes in the IFA test. The recombinant antigen was detected by antibodies to 3 serotypes of hantaviruses (Hantaan 76-118, SR-11, Puumala). Antibody titers of a group of experimentally infected mouse and urban rat sera using rNP-SR-Sf9 and strain SR-11-infected Vero cell antigens were mutual correlative. These results indicated that the rNP-SR-Sf9 were an effective and safety substitute for Vero E6 cells in the IFA test for serosurveys of hantavirus infection.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, Viral / analysis*
  • Baculoviridae / genetics*
  • Capsid / analysis*
  • Capsid / biosynthesis
  • Cell Line
  • Cross Reactions
  • Fluorescent Antibody Technique*
  • Hemorrhagic Fever with Renal Syndrome / diagnosis*
  • Kidney Diseases / microbiology*
  • Mice
  • Moths
  • Orthohantavirus / genetics
  • Orthohantavirus / isolation & purification*
  • Orthohantavirus / metabolism
  • Rats
  • Syndrome
  • Vero Cells

Substances

  • Antigens, Viral