Stability of DNA fingerprint pattern produced with IS6110 in strains of Mycobacterium tuberculosis

J Clin Microbiol. 1994 Jan;32(1):262-6. doi: 10.1128/jcm.32.1.262-266.1994.

Abstract

To assess the stability of IS6110 restriction fragment length polymorphism patterns, DNA fingerprints of 6 Mycobacterium bovis isolates from 1 patient and of 41 Mycobacterium tuberculosis isolates from 18 patients were compared. The fingerprint pattern for a given patient remained identical or nearly identical despite recovery of the isolates during intervals which ranged from 8 months to 4.5 years. Changes in drug resistance profile did not alter a strain's fingerprint pattern.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • DNA Fingerprinting
  • DNA Transposable Elements / genetics*
  • DNA, Bacterial / genetics*
  • Drug Resistance, Microbial / genetics
  • Genome, Bacterial
  • Humans
  • Mycobacterium tuberculosis / genetics*
  • Mycobacterium tuberculosis / isolation & purification
  • Polymorphism, Restriction Fragment Length*
  • Species Specificity
  • Sputum / microbiology
  • Tuberculosis / microbiology*
  • Urine / microbiology

Substances

  • DNA Transposable Elements
  • DNA, Bacterial